oligo dt 65 Search Results


oligo dt  (TaKaRa)
94
TaKaRa oligo dt
Oligo Dt, supplied by TaKaRa, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 94 stars, based on 1 article reviews
oligo dt - by Bioz Stars, 2026-04
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oligo dt 65  (Integrated DNA Technologies)
94
Integrated DNA Technologies oligo dt 65
Effects of DNA in solution and DNA incorporated into a FITC-fibrin film on fibrinolysis. A, changes in the fluorescence emission at 520 nm (excitation 493 nm) during the incubation of FITC-fibrin film with 100 μl of a mixture of 100 nm Glu-Plg with 0.25 nm tctPA without DNA (●) and with 1.0 (○) and 25 (□) μg/ml dsDNA in 0.05 m Hepes/NaOH buffer (pH 7.4) (with 20 mm NaCl and BSA 1 mg/ml). Inset, dependences of rates of fibrinolysis of FITC-fibrin film by 20 nm PL (Δ), mixtures of 100 nm Glu-Plg with 0.25 nm tctPA (●), or 0.25 nm tcuPA (○), 100 nm Plg/tctPA with 15 nm α2AP (■) and 100 nm Plg/tcuPA with 15 nm α2AP (□) on the concentration of dsDNA in the solution. The rates of fibrinolysis were calculated as slopes of a linear (r2 >0.9) increase in the fluorescein fluorescence emission with time (PL) or the square of time (tctPA/Plg and tcuPA/Plg) and expressed as the % of the control reaction (no DNA added). B, effects of dsDNA incorporated into a FITC-fibrin film on the rates of fibrinolysis by 20 nm PL (Δ) and a mixture of 100 nm Glu-Plg with 0.25 nm tctPA (●) or 0.25 nm tcuPA (○). The indicated amounts of dsDNA (μg/well) were added to 20 μg of FITC-Fbg before its polymerization. The rates of fibrinolysis were calculated as slopes of a linear (r2 >0.9) increase in the fluorescein fluorescence emission with time (PL) or the square of time (tctPA/Plg) and expressed as % of the control reaction (no DNA added). C, effects of <t>oligo(dT)65</t> incorporated into FITC-fibrin film on the rates of fibrinolysis by 20 nm PL (Δ) and a mixture of 100 nm Glu-Plg with 0.25 nm tctPA (●) or 0.25 nm tcuPA (○). The indicated amounts of nanomoles of oligo(dT)65 per well were added to 20 μg of FITC-Fbg before its polymerization. Rates of fibrinolysis were calculated as slopes of a linear (r2 >0.9) increase in the fluorescein fluorescence emission with time (PL) or the square of time (tctPA/Plg) and expressed as % of the control reaction (no oligo(dT)65 added). D, effects of α2AP (15 nm) on the rates of fibrinolysis of FITC-fibrin films with incorporated dsDNA (circles) or oligo(dT)65 (squares) by 100 nm Plg/tctPA (filled symbols) and 100 nm Plg/tcuPA (open symbols). The indicated amounts of dsDNA (μg/well) or oligo(dT)65 (nanomoles/well) were added to 20 μg of FITC-Fbg before its polymerization. The rates of fibrinolysis were calculated as slopes of a linear (r2 >0.9) increase in the fluorescein fluorescence emission with the square of time and expressed as % of the control reaction (no dsDNA or oligo(dT)65 added).
Oligo Dt 65, supplied by Integrated DNA Technologies, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 94 stars, based on 1 article reviews
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poly a enrichment with oligo dt 25 magnetic beads  (New England Biolabs)
96
New England Biolabs poly a enrichment with oligo dt 25 magnetic beads
Effects of DNA in solution and DNA incorporated into a FITC-fibrin film on fibrinolysis. A, changes in the fluorescence emission at 520 nm (excitation 493 nm) during the incubation of FITC-fibrin film with 100 μl of a mixture of 100 nm Glu-Plg with 0.25 nm tctPA without DNA (●) and with 1.0 (○) and 25 (□) μg/ml dsDNA in 0.05 m Hepes/NaOH buffer (pH 7.4) (with 20 mm NaCl and BSA 1 mg/ml). Inset, dependences of rates of fibrinolysis of FITC-fibrin film by 20 nm PL (Δ), mixtures of 100 nm Glu-Plg with 0.25 nm tctPA (●), or 0.25 nm tcuPA (○), 100 nm Plg/tctPA with 15 nm α2AP (■) and 100 nm Plg/tcuPA with 15 nm α2AP (□) on the concentration of dsDNA in the solution. The rates of fibrinolysis were calculated as slopes of a linear (r2 >0.9) increase in the fluorescein fluorescence emission with time (PL) or the square of time (tctPA/Plg and tcuPA/Plg) and expressed as the % of the control reaction (no DNA added). B, effects of dsDNA incorporated into a FITC-fibrin film on the rates of fibrinolysis by 20 nm PL (Δ) and a mixture of 100 nm Glu-Plg with 0.25 nm tctPA (●) or 0.25 nm tcuPA (○). The indicated amounts of dsDNA (μg/well) were added to 20 μg of FITC-Fbg before its polymerization. The rates of fibrinolysis were calculated as slopes of a linear (r2 >0.9) increase in the fluorescein fluorescence emission with time (PL) or the square of time (tctPA/Plg) and expressed as % of the control reaction (no DNA added). C, effects of <t>oligo(dT)65</t> incorporated into FITC-fibrin film on the rates of fibrinolysis by 20 nm PL (Δ) and a mixture of 100 nm Glu-Plg with 0.25 nm tctPA (●) or 0.25 nm tcuPA (○). The indicated amounts of nanomoles of oligo(dT)65 per well were added to 20 μg of FITC-Fbg before its polymerization. Rates of fibrinolysis were calculated as slopes of a linear (r2 >0.9) increase in the fluorescein fluorescence emission with time (PL) or the square of time (tctPA/Plg) and expressed as % of the control reaction (no oligo(dT)65 added). D, effects of α2AP (15 nm) on the rates of fibrinolysis of FITC-fibrin films with incorporated dsDNA (circles) or oligo(dT)65 (squares) by 100 nm Plg/tctPA (filled symbols) and 100 nm Plg/tcuPA (open symbols). The indicated amounts of dsDNA (μg/well) or oligo(dT)65 (nanomoles/well) were added to 20 μg of FITC-Fbg before its polymerization. The rates of fibrinolysis were calculated as slopes of a linear (r2 >0.9) increase in the fluorescein fluorescence emission with the square of time and expressed as % of the control reaction (no dsDNA or oligo(dT)65 added).
Poly A Enrichment With Oligo Dt 25 Magnetic Beads, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 96 stars, based on 1 article reviews
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oligo(dt  (Thermo Fisher)
90
Thermo Fisher oligo(dt
Effects of DNA in solution and DNA incorporated into a FITC-fibrin film on fibrinolysis. A, changes in the fluorescence emission at 520 nm (excitation 493 nm) during the incubation of FITC-fibrin film with 100 μl of a mixture of 100 nm Glu-Plg with 0.25 nm tctPA without DNA (●) and with 1.0 (○) and 25 (□) μg/ml dsDNA in 0.05 m Hepes/NaOH buffer (pH 7.4) (with 20 mm NaCl and BSA 1 mg/ml). Inset, dependences of rates of fibrinolysis of FITC-fibrin film by 20 nm PL (Δ), mixtures of 100 nm Glu-Plg with 0.25 nm tctPA (●), or 0.25 nm tcuPA (○), 100 nm Plg/tctPA with 15 nm α2AP (■) and 100 nm Plg/tcuPA with 15 nm α2AP (□) on the concentration of dsDNA in the solution. The rates of fibrinolysis were calculated as slopes of a linear (r2 >0.9) increase in the fluorescein fluorescence emission with time (PL) or the square of time (tctPA/Plg and tcuPA/Plg) and expressed as the % of the control reaction (no DNA added). B, effects of dsDNA incorporated into a FITC-fibrin film on the rates of fibrinolysis by 20 nm PL (Δ) and a mixture of 100 nm Glu-Plg with 0.25 nm tctPA (●) or 0.25 nm tcuPA (○). The indicated amounts of dsDNA (μg/well) were added to 20 μg of FITC-Fbg before its polymerization. The rates of fibrinolysis were calculated as slopes of a linear (r2 >0.9) increase in the fluorescein fluorescence emission with time (PL) or the square of time (tctPA/Plg) and expressed as % of the control reaction (no DNA added). C, effects of <t>oligo(dT)65</t> incorporated into FITC-fibrin film on the rates of fibrinolysis by 20 nm PL (Δ) and a mixture of 100 nm Glu-Plg with 0.25 nm tctPA (●) or 0.25 nm tcuPA (○). The indicated amounts of nanomoles of oligo(dT)65 per well were added to 20 μg of FITC-Fbg before its polymerization. Rates of fibrinolysis were calculated as slopes of a linear (r2 >0.9) increase in the fluorescein fluorescence emission with time (PL) or the square of time (tctPA/Plg) and expressed as % of the control reaction (no oligo(dT)65 added). D, effects of α2AP (15 nm) on the rates of fibrinolysis of FITC-fibrin films with incorporated dsDNA (circles) or oligo(dT)65 (squares) by 100 nm Plg/tctPA (filled symbols) and 100 nm Plg/tcuPA (open symbols). The indicated amounts of dsDNA (μg/well) or oligo(dT)65 (nanomoles/well) were added to 20 μg of FITC-Fbg before its polymerization. The rates of fibrinolysis were calculated as slopes of a linear (r2 >0.9) increase in the fluorescein fluorescence emission with the square of time and expressed as % of the control reaction (no dsDNA or oligo(dT)65 added).
Oligo(Dt, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 90 stars, based on 1 article reviews
oligo(dt - by Bioz Stars, 2026-04
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rnasin plus rnase inhibitor  (Promega)
90
Promega rnasin plus rnase inhibitor
Effects of DNA in solution and DNA incorporated into a FITC-fibrin film on fibrinolysis. A, changes in the fluorescence emission at 520 nm (excitation 493 nm) during the incubation of FITC-fibrin film with 100 μl of a mixture of 100 nm Glu-Plg with 0.25 nm tctPA without DNA (●) and with 1.0 (○) and 25 (□) μg/ml dsDNA in 0.05 m Hepes/NaOH buffer (pH 7.4) (with 20 mm NaCl and BSA 1 mg/ml). Inset, dependences of rates of fibrinolysis of FITC-fibrin film by 20 nm PL (Δ), mixtures of 100 nm Glu-Plg with 0.25 nm tctPA (●), or 0.25 nm tcuPA (○), 100 nm Plg/tctPA with 15 nm α2AP (■) and 100 nm Plg/tcuPA with 15 nm α2AP (□) on the concentration of dsDNA in the solution. The rates of fibrinolysis were calculated as slopes of a linear (r2 >0.9) increase in the fluorescein fluorescence emission with time (PL) or the square of time (tctPA/Plg and tcuPA/Plg) and expressed as the % of the control reaction (no DNA added). B, effects of dsDNA incorporated into a FITC-fibrin film on the rates of fibrinolysis by 20 nm PL (Δ) and a mixture of 100 nm Glu-Plg with 0.25 nm tctPA (●) or 0.25 nm tcuPA (○). The indicated amounts of dsDNA (μg/well) were added to 20 μg of FITC-Fbg before its polymerization. The rates of fibrinolysis were calculated as slopes of a linear (r2 >0.9) increase in the fluorescein fluorescence emission with time (PL) or the square of time (tctPA/Plg) and expressed as % of the control reaction (no DNA added). C, effects of <t>oligo(dT)65</t> incorporated into FITC-fibrin film on the rates of fibrinolysis by 20 nm PL (Δ) and a mixture of 100 nm Glu-Plg with 0.25 nm tctPA (●) or 0.25 nm tcuPA (○). The indicated amounts of nanomoles of oligo(dT)65 per well were added to 20 μg of FITC-Fbg before its polymerization. Rates of fibrinolysis were calculated as slopes of a linear (r2 >0.9) increase in the fluorescein fluorescence emission with time (PL) or the square of time (tctPA/Plg) and expressed as % of the control reaction (no oligo(dT)65 added). D, effects of α2AP (15 nm) on the rates of fibrinolysis of FITC-fibrin films with incorporated dsDNA (circles) or oligo(dT)65 (squares) by 100 nm Plg/tctPA (filled symbols) and 100 nm Plg/tcuPA (open symbols). The indicated amounts of dsDNA (μg/well) or oligo(dT)65 (nanomoles/well) were added to 20 μg of FITC-Fbg before its polymerization. The rates of fibrinolysis were calculated as slopes of a linear (r2 >0.9) increase in the fluorescein fluorescence emission with the square of time and expressed as % of the control reaction (no dsDNA or oligo(dT)65 added).
Rnasin Plus Rnase Inhibitor, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 90 stars, based on 1 article reviews
rnasin plus rnase inhibitor - by Bioz Stars, 2026-04
90/100 stars
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oligo dt  (Thermo Fisher)
99
Thermo Fisher oligo dt
Effects of DNA in solution and DNA incorporated into a FITC-fibrin film on fibrinolysis. A, changes in the fluorescence emission at 520 nm (excitation 493 nm) during the incubation of FITC-fibrin film with 100 μl of a mixture of 100 nm Glu-Plg with 0.25 nm tctPA without DNA (●) and with 1.0 (○) and 25 (□) μg/ml dsDNA in 0.05 m Hepes/NaOH buffer (pH 7.4) (with 20 mm NaCl and BSA 1 mg/ml). Inset, dependences of rates of fibrinolysis of FITC-fibrin film by 20 nm PL (Δ), mixtures of 100 nm Glu-Plg with 0.25 nm tctPA (●), or 0.25 nm tcuPA (○), 100 nm Plg/tctPA with 15 nm α2AP (■) and 100 nm Plg/tcuPA with 15 nm α2AP (□) on the concentration of dsDNA in the solution. The rates of fibrinolysis were calculated as slopes of a linear (r2 >0.9) increase in the fluorescein fluorescence emission with time (PL) or the square of time (tctPA/Plg and tcuPA/Plg) and expressed as the % of the control reaction (no DNA added). B, effects of dsDNA incorporated into a FITC-fibrin film on the rates of fibrinolysis by 20 nm PL (Δ) and a mixture of 100 nm Glu-Plg with 0.25 nm tctPA (●) or 0.25 nm tcuPA (○). The indicated amounts of dsDNA (μg/well) were added to 20 μg of FITC-Fbg before its polymerization. The rates of fibrinolysis were calculated as slopes of a linear (r2 >0.9) increase in the fluorescein fluorescence emission with time (PL) or the square of time (tctPA/Plg) and expressed as % of the control reaction (no DNA added). C, effects of <t>oligo(dT)65</t> incorporated into FITC-fibrin film on the rates of fibrinolysis by 20 nm PL (Δ) and a mixture of 100 nm Glu-Plg with 0.25 nm tctPA (●) or 0.25 nm tcuPA (○). The indicated amounts of nanomoles of oligo(dT)65 per well were added to 20 μg of FITC-Fbg before its polymerization. Rates of fibrinolysis were calculated as slopes of a linear (r2 >0.9) increase in the fluorescein fluorescence emission with time (PL) or the square of time (tctPA/Plg) and expressed as % of the control reaction (no oligo(dT)65 added). D, effects of α2AP (15 nm) on the rates of fibrinolysis of FITC-fibrin films with incorporated dsDNA (circles) or oligo(dT)65 (squares) by 100 nm Plg/tctPA (filled symbols) and 100 nm Plg/tcuPA (open symbols). The indicated amounts of dsDNA (μg/well) or oligo(dT)65 (nanomoles/well) were added to 20 μg of FITC-Fbg before its polymerization. The rates of fibrinolysis were calculated as slopes of a linear (r2 >0.9) increase in the fluorescein fluorescence emission with the square of time and expressed as % of the control reaction (no dsDNA or oligo(dT)65 added).
Oligo Dt, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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1 μg of oligo(dt) or random primers  (Thermo Fisher)
90
Thermo Fisher 1 μg of oligo(dt) or random primers
Effects of DNA in solution and DNA incorporated into a FITC-fibrin film on fibrinolysis. A, changes in the fluorescence emission at 520 nm (excitation 493 nm) during the incubation of FITC-fibrin film with 100 μl of a mixture of 100 nm Glu-Plg with 0.25 nm tctPA without DNA (●) and with 1.0 (○) and 25 (□) μg/ml dsDNA in 0.05 m Hepes/NaOH buffer (pH 7.4) (with 20 mm NaCl and BSA 1 mg/ml). Inset, dependences of rates of fibrinolysis of FITC-fibrin film by 20 nm PL (Δ), mixtures of 100 nm Glu-Plg with 0.25 nm tctPA (●), or 0.25 nm tcuPA (○), 100 nm Plg/tctPA with 15 nm α2AP (■) and 100 nm Plg/tcuPA with 15 nm α2AP (□) on the concentration of dsDNA in the solution. The rates of fibrinolysis were calculated as slopes of a linear (r2 >0.9) increase in the fluorescein fluorescence emission with time (PL) or the square of time (tctPA/Plg and tcuPA/Plg) and expressed as the % of the control reaction (no DNA added). B, effects of dsDNA incorporated into a FITC-fibrin film on the rates of fibrinolysis by 20 nm PL (Δ) and a mixture of 100 nm Glu-Plg with 0.25 nm tctPA (●) or 0.25 nm tcuPA (○). The indicated amounts of dsDNA (μg/well) were added to 20 μg of FITC-Fbg before its polymerization. The rates of fibrinolysis were calculated as slopes of a linear (r2 >0.9) increase in the fluorescein fluorescence emission with time (PL) or the square of time (tctPA/Plg) and expressed as % of the control reaction (no DNA added). C, effects of <t>oligo(dT)65</t> incorporated into FITC-fibrin film on the rates of fibrinolysis by 20 nm PL (Δ) and a mixture of 100 nm Glu-Plg with 0.25 nm tctPA (●) or 0.25 nm tcuPA (○). The indicated amounts of nanomoles of oligo(dT)65 per well were added to 20 μg of FITC-Fbg before its polymerization. Rates of fibrinolysis were calculated as slopes of a linear (r2 >0.9) increase in the fluorescein fluorescence emission with time (PL) or the square of time (tctPA/Plg) and expressed as % of the control reaction (no oligo(dT)65 added). D, effects of α2AP (15 nm) on the rates of fibrinolysis of FITC-fibrin films with incorporated dsDNA (circles) or oligo(dT)65 (squares) by 100 nm Plg/tctPA (filled symbols) and 100 nm Plg/tcuPA (open symbols). The indicated amounts of dsDNA (μg/well) or oligo(dT)65 (nanomoles/well) were added to 20 μg of FITC-Fbg before its polymerization. The rates of fibrinolysis were calculated as slopes of a linear (r2 >0.9) increase in the fluorescein fluorescence emission with the square of time and expressed as % of the control reaction (no dsDNA or oligo(dT)65 added).
1 μg Of Oligo(Dt) Or Random Primers, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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1 μg of oligo(dt) or random primers - by Bioz Stars, 2026-04
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oligo(dt)15 primers  (Promega)
90
Promega oligo(dt)15 primers
Effects of DNA in solution and DNA incorporated into a FITC-fibrin film on fibrinolysis. A, changes in the fluorescence emission at 520 nm (excitation 493 nm) during the incubation of FITC-fibrin film with 100 μl of a mixture of 100 nm Glu-Plg with 0.25 nm tctPA without DNA (●) and with 1.0 (○) and 25 (□) μg/ml dsDNA in 0.05 m Hepes/NaOH buffer (pH 7.4) (with 20 mm NaCl and BSA 1 mg/ml). Inset, dependences of rates of fibrinolysis of FITC-fibrin film by 20 nm PL (Δ), mixtures of 100 nm Glu-Plg with 0.25 nm tctPA (●), or 0.25 nm tcuPA (○), 100 nm Plg/tctPA with 15 nm α2AP (■) and 100 nm Plg/tcuPA with 15 nm α2AP (□) on the concentration of dsDNA in the solution. The rates of fibrinolysis were calculated as slopes of a linear (r2 >0.9) increase in the fluorescein fluorescence emission with time (PL) or the square of time (tctPA/Plg and tcuPA/Plg) and expressed as the % of the control reaction (no DNA added). B, effects of dsDNA incorporated into a FITC-fibrin film on the rates of fibrinolysis by 20 nm PL (Δ) and a mixture of 100 nm Glu-Plg with 0.25 nm tctPA (●) or 0.25 nm tcuPA (○). The indicated amounts of dsDNA (μg/well) were added to 20 μg of FITC-Fbg before its polymerization. The rates of fibrinolysis were calculated as slopes of a linear (r2 >0.9) increase in the fluorescein fluorescence emission with time (PL) or the square of time (tctPA/Plg) and expressed as % of the control reaction (no DNA added). C, effects of <t>oligo(dT)65</t> incorporated into FITC-fibrin film on the rates of fibrinolysis by 20 nm PL (Δ) and a mixture of 100 nm Glu-Plg with 0.25 nm tctPA (●) or 0.25 nm tcuPA (○). The indicated amounts of nanomoles of oligo(dT)65 per well were added to 20 μg of FITC-Fbg before its polymerization. Rates of fibrinolysis were calculated as slopes of a linear (r2 >0.9) increase in the fluorescein fluorescence emission with time (PL) or the square of time (tctPA/Plg) and expressed as % of the control reaction (no oligo(dT)65 added). D, effects of α2AP (15 nm) on the rates of fibrinolysis of FITC-fibrin films with incorporated dsDNA (circles) or oligo(dT)65 (squares) by 100 nm Plg/tctPA (filled symbols) and 100 nm Plg/tcuPA (open symbols). The indicated amounts of dsDNA (μg/well) or oligo(dT)65 (nanomoles/well) were added to 20 μg of FITC-Fbg before its polymerization. The rates of fibrinolysis were calculated as slopes of a linear (r2 >0.9) increase in the fluorescein fluorescence emission with the square of time and expressed as % of the control reaction (no dsDNA or oligo(dT)65 added).
Oligo(Dt)15 Primers, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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oligo(dt)15 primers - by Bioz Stars, 2026-04
90/100 stars
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oligo(dt)  (Promega)
90
Promega oligo(dt)
Effects of DNA in solution and DNA incorporated into a FITC-fibrin film on fibrinolysis. A, changes in the fluorescence emission at 520 nm (excitation 493 nm) during the incubation of FITC-fibrin film with 100 μl of a mixture of 100 nm Glu-Plg with 0.25 nm tctPA without DNA (●) and with 1.0 (○) and 25 (□) μg/ml dsDNA in 0.05 m Hepes/NaOH buffer (pH 7.4) (with 20 mm NaCl and BSA 1 mg/ml). Inset, dependences of rates of fibrinolysis of FITC-fibrin film by 20 nm PL (Δ), mixtures of 100 nm Glu-Plg with 0.25 nm tctPA (●), or 0.25 nm tcuPA (○), 100 nm Plg/tctPA with 15 nm α2AP (■) and 100 nm Plg/tcuPA with 15 nm α2AP (□) on the concentration of dsDNA in the solution. The rates of fibrinolysis were calculated as slopes of a linear (r2 >0.9) increase in the fluorescein fluorescence emission with time (PL) or the square of time (tctPA/Plg and tcuPA/Plg) and expressed as the % of the control reaction (no DNA added). B, effects of dsDNA incorporated into a FITC-fibrin film on the rates of fibrinolysis by 20 nm PL (Δ) and a mixture of 100 nm Glu-Plg with 0.25 nm tctPA (●) or 0.25 nm tcuPA (○). The indicated amounts of dsDNA (μg/well) were added to 20 μg of FITC-Fbg before its polymerization. The rates of fibrinolysis were calculated as slopes of a linear (r2 >0.9) increase in the fluorescein fluorescence emission with time (PL) or the square of time (tctPA/Plg) and expressed as % of the control reaction (no DNA added). C, effects of <t>oligo(dT)65</t> incorporated into FITC-fibrin film on the rates of fibrinolysis by 20 nm PL (Δ) and a mixture of 100 nm Glu-Plg with 0.25 nm tctPA (●) or 0.25 nm tcuPA (○). The indicated amounts of nanomoles of oligo(dT)65 per well were added to 20 μg of FITC-Fbg before its polymerization. Rates of fibrinolysis were calculated as slopes of a linear (r2 >0.9) increase in the fluorescein fluorescence emission with time (PL) or the square of time (tctPA/Plg) and expressed as % of the control reaction (no oligo(dT)65 added). D, effects of α2AP (15 nm) on the rates of fibrinolysis of FITC-fibrin films with incorporated dsDNA (circles) or oligo(dT)65 (squares) by 100 nm Plg/tctPA (filled symbols) and 100 nm Plg/tcuPA (open symbols). The indicated amounts of dsDNA (μg/well) or oligo(dT)65 (nanomoles/well) were added to 20 μg of FITC-Fbg before its polymerization. The rates of fibrinolysis were calculated as slopes of a linear (r2 >0.9) increase in the fluorescein fluorescence emission with the square of time and expressed as % of the control reaction (no dsDNA or oligo(dT)65 added).
Oligo(Dt), supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 90 stars, based on 1 article reviews
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oligo(dt) primers  (Thermo Fisher)
90
Thermo Fisher oligo(dt) primers
Effects of DNA in solution and DNA incorporated into a FITC-fibrin film on fibrinolysis. A, changes in the fluorescence emission at 520 nm (excitation 493 nm) during the incubation of FITC-fibrin film with 100 μl of a mixture of 100 nm Glu-Plg with 0.25 nm tctPA without DNA (●) and with 1.0 (○) and 25 (□) μg/ml dsDNA in 0.05 m Hepes/NaOH buffer (pH 7.4) (with 20 mm NaCl and BSA 1 mg/ml). Inset, dependences of rates of fibrinolysis of FITC-fibrin film by 20 nm PL (Δ), mixtures of 100 nm Glu-Plg with 0.25 nm tctPA (●), or 0.25 nm tcuPA (○), 100 nm Plg/tctPA with 15 nm α2AP (■) and 100 nm Plg/tcuPA with 15 nm α2AP (□) on the concentration of dsDNA in the solution. The rates of fibrinolysis were calculated as slopes of a linear (r2 >0.9) increase in the fluorescein fluorescence emission with time (PL) or the square of time (tctPA/Plg and tcuPA/Plg) and expressed as the % of the control reaction (no DNA added). B, effects of dsDNA incorporated into a FITC-fibrin film on the rates of fibrinolysis by 20 nm PL (Δ) and a mixture of 100 nm Glu-Plg with 0.25 nm tctPA (●) or 0.25 nm tcuPA (○). The indicated amounts of dsDNA (μg/well) were added to 20 μg of FITC-Fbg before its polymerization. The rates of fibrinolysis were calculated as slopes of a linear (r2 >0.9) increase in the fluorescein fluorescence emission with time (PL) or the square of time (tctPA/Plg) and expressed as % of the control reaction (no DNA added). C, effects of <t>oligo(dT)65</t> incorporated into FITC-fibrin film on the rates of fibrinolysis by 20 nm PL (Δ) and a mixture of 100 nm Glu-Plg with 0.25 nm tctPA (●) or 0.25 nm tcuPA (○). The indicated amounts of nanomoles of oligo(dT)65 per well were added to 20 μg of FITC-Fbg before its polymerization. Rates of fibrinolysis were calculated as slopes of a linear (r2 >0.9) increase in the fluorescein fluorescence emission with time (PL) or the square of time (tctPA/Plg) and expressed as % of the control reaction (no oligo(dT)65 added). D, effects of α2AP (15 nm) on the rates of fibrinolysis of FITC-fibrin films with incorporated dsDNA (circles) or oligo(dT)65 (squares) by 100 nm Plg/tctPA (filled symbols) and 100 nm Plg/tcuPA (open symbols). The indicated amounts of dsDNA (μg/well) or oligo(dT)65 (nanomoles/well) were added to 20 μg of FITC-Fbg before its polymerization. The rates of fibrinolysis were calculated as slopes of a linear (r2 >0.9) increase in the fluorescein fluorescence emission with the square of time and expressed as % of the control reaction (no dsDNA or oligo(dT)65 added).
Oligo(Dt) Primers, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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oligo(dt)15 primer  (Promega)
90
Promega oligo(dt)15 primer
Effects of DNA in solution and DNA incorporated into a FITC-fibrin film on fibrinolysis. A, changes in the fluorescence emission at 520 nm (excitation 493 nm) during the incubation of FITC-fibrin film with 100 μl of a mixture of 100 nm Glu-Plg with 0.25 nm tctPA without DNA (●) and with 1.0 (○) and 25 (□) μg/ml dsDNA in 0.05 m Hepes/NaOH buffer (pH 7.4) (with 20 mm NaCl and BSA 1 mg/ml). Inset, dependences of rates of fibrinolysis of FITC-fibrin film by 20 nm PL (Δ), mixtures of 100 nm Glu-Plg with 0.25 nm tctPA (●), or 0.25 nm tcuPA (○), 100 nm Plg/tctPA with 15 nm α2AP (■) and 100 nm Plg/tcuPA with 15 nm α2AP (□) on the concentration of dsDNA in the solution. The rates of fibrinolysis were calculated as slopes of a linear (r2 >0.9) increase in the fluorescein fluorescence emission with time (PL) or the square of time (tctPA/Plg and tcuPA/Plg) and expressed as the % of the control reaction (no DNA added). B, effects of dsDNA incorporated into a FITC-fibrin film on the rates of fibrinolysis by 20 nm PL (Δ) and a mixture of 100 nm Glu-Plg with 0.25 nm tctPA (●) or 0.25 nm tcuPA (○). The indicated amounts of dsDNA (μg/well) were added to 20 μg of FITC-Fbg before its polymerization. The rates of fibrinolysis were calculated as slopes of a linear (r2 >0.9) increase in the fluorescein fluorescence emission with time (PL) or the square of time (tctPA/Plg) and expressed as % of the control reaction (no DNA added). C, effects of <t>oligo(dT)65</t> incorporated into FITC-fibrin film on the rates of fibrinolysis by 20 nm PL (Δ) and a mixture of 100 nm Glu-Plg with 0.25 nm tctPA (●) or 0.25 nm tcuPA (○). The indicated amounts of nanomoles of oligo(dT)65 per well were added to 20 μg of FITC-Fbg before its polymerization. Rates of fibrinolysis were calculated as slopes of a linear (r2 >0.9) increase in the fluorescein fluorescence emission with time (PL) or the square of time (tctPA/Plg) and expressed as % of the control reaction (no oligo(dT)65 added). D, effects of α2AP (15 nm) on the rates of fibrinolysis of FITC-fibrin films with incorporated dsDNA (circles) or oligo(dT)65 (squares) by 100 nm Plg/tctPA (filled symbols) and 100 nm Plg/tcuPA (open symbols). The indicated amounts of dsDNA (μg/well) or oligo(dT)65 (nanomoles/well) were added to 20 μg of FITC-Fbg before its polymerization. The rates of fibrinolysis were calculated as slopes of a linear (r2 >0.9) increase in the fluorescein fluorescence emission with the square of time and expressed as % of the control reaction (no dsDNA or oligo(dT)65 added).
Oligo(Dt)15 Primer, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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biotinylated-oligo (dt) probe  (Promega)
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Promega biotinylated-oligo (dt) probe
Effects of DNA in solution and DNA incorporated into a FITC-fibrin film on fibrinolysis. A, changes in the fluorescence emission at 520 nm (excitation 493 nm) during the incubation of FITC-fibrin film with 100 μl of a mixture of 100 nm Glu-Plg with 0.25 nm tctPA without DNA (●) and with 1.0 (○) and 25 (□) μg/ml dsDNA in 0.05 m Hepes/NaOH buffer (pH 7.4) (with 20 mm NaCl and BSA 1 mg/ml). Inset, dependences of rates of fibrinolysis of FITC-fibrin film by 20 nm PL (Δ), mixtures of 100 nm Glu-Plg with 0.25 nm tctPA (●), or 0.25 nm tcuPA (○), 100 nm Plg/tctPA with 15 nm α2AP (■) and 100 nm Plg/tcuPA with 15 nm α2AP (□) on the concentration of dsDNA in the solution. The rates of fibrinolysis were calculated as slopes of a linear (r2 >0.9) increase in the fluorescein fluorescence emission with time (PL) or the square of time (tctPA/Plg and tcuPA/Plg) and expressed as the % of the control reaction (no DNA added). B, effects of dsDNA incorporated into a FITC-fibrin film on the rates of fibrinolysis by 20 nm PL (Δ) and a mixture of 100 nm Glu-Plg with 0.25 nm tctPA (●) or 0.25 nm tcuPA (○). The indicated amounts of dsDNA (μg/well) were added to 20 μg of FITC-Fbg before its polymerization. The rates of fibrinolysis were calculated as slopes of a linear (r2 >0.9) increase in the fluorescein fluorescence emission with time (PL) or the square of time (tctPA/Plg) and expressed as % of the control reaction (no DNA added). C, effects of <t>oligo(dT)65</t> incorporated into FITC-fibrin film on the rates of fibrinolysis by 20 nm PL (Δ) and a mixture of 100 nm Glu-Plg with 0.25 nm tctPA (●) or 0.25 nm tcuPA (○). The indicated amounts of nanomoles of oligo(dT)65 per well were added to 20 μg of FITC-Fbg before its polymerization. Rates of fibrinolysis were calculated as slopes of a linear (r2 >0.9) increase in the fluorescein fluorescence emission with time (PL) or the square of time (tctPA/Plg) and expressed as % of the control reaction (no oligo(dT)65 added). D, effects of α2AP (15 nm) on the rates of fibrinolysis of FITC-fibrin films with incorporated dsDNA (circles) or oligo(dT)65 (squares) by 100 nm Plg/tctPA (filled symbols) and 100 nm Plg/tcuPA (open symbols). The indicated amounts of dsDNA (μg/well) or oligo(dT)65 (nanomoles/well) were added to 20 μg of FITC-Fbg before its polymerization. The rates of fibrinolysis were calculated as slopes of a linear (r2 >0.9) increase in the fluorescein fluorescence emission with the square of time and expressed as % of the control reaction (no dsDNA or oligo(dT)65 added).
Biotinylated Oligo (Dt) Probe, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Effects of DNA in solution and DNA incorporated into a FITC-fibrin film on fibrinolysis. A, changes in the fluorescence emission at 520 nm (excitation 493 nm) during the incubation of FITC-fibrin film with 100 μl of a mixture of 100 nm Glu-Plg with 0.25 nm tctPA without DNA (●) and with 1.0 (○) and 25 (□) μg/ml dsDNA in 0.05 m Hepes/NaOH buffer (pH 7.4) (with 20 mm NaCl and BSA 1 mg/ml). Inset, dependences of rates of fibrinolysis of FITC-fibrin film by 20 nm PL (Δ), mixtures of 100 nm Glu-Plg with 0.25 nm tctPA (●), or 0.25 nm tcuPA (○), 100 nm Plg/tctPA with 15 nm α2AP (■) and 100 nm Plg/tcuPA with 15 nm α2AP (□) on the concentration of dsDNA in the solution. The rates of fibrinolysis were calculated as slopes of a linear (r2 >0.9) increase in the fluorescein fluorescence emission with time (PL) or the square of time (tctPA/Plg and tcuPA/Plg) and expressed as the % of the control reaction (no DNA added). B, effects of dsDNA incorporated into a FITC-fibrin film on the rates of fibrinolysis by 20 nm PL (Δ) and a mixture of 100 nm Glu-Plg with 0.25 nm tctPA (●) or 0.25 nm tcuPA (○). The indicated amounts of dsDNA (μg/well) were added to 20 μg of FITC-Fbg before its polymerization. The rates of fibrinolysis were calculated as slopes of a linear (r2 >0.9) increase in the fluorescein fluorescence emission with time (PL) or the square of time (tctPA/Plg) and expressed as % of the control reaction (no DNA added). C, effects of oligo(dT)65 incorporated into FITC-fibrin film on the rates of fibrinolysis by 20 nm PL (Δ) and a mixture of 100 nm Glu-Plg with 0.25 nm tctPA (●) or 0.25 nm tcuPA (○). The indicated amounts of nanomoles of oligo(dT)65 per well were added to 20 μg of FITC-Fbg before its polymerization. Rates of fibrinolysis were calculated as slopes of a linear (r2 >0.9) increase in the fluorescein fluorescence emission with time (PL) or the square of time (tctPA/Plg) and expressed as % of the control reaction (no oligo(dT)65 added). D, effects of α2AP (15 nm) on the rates of fibrinolysis of FITC-fibrin films with incorporated dsDNA (circles) or oligo(dT)65 (squares) by 100 nm Plg/tctPA (filled symbols) and 100 nm Plg/tcuPA (open symbols). The indicated amounts of dsDNA (μg/well) or oligo(dT)65 (nanomoles/well) were added to 20 μg of FITC-Fbg before its polymerization. The rates of fibrinolysis were calculated as slopes of a linear (r2 >0.9) increase in the fluorescein fluorescence emission with the square of time and expressed as % of the control reaction (no dsDNA or oligo(dT)65 added).

Journal: The Journal of Biological Chemistry

Article Title: Effects of Extracellular DNA on Plasminogen Activation and Fibrinolysis *

doi: 10.1074/jbc.M111.301218

Figure Lengend Snippet: Effects of DNA in solution and DNA incorporated into a FITC-fibrin film on fibrinolysis. A, changes in the fluorescence emission at 520 nm (excitation 493 nm) during the incubation of FITC-fibrin film with 100 μl of a mixture of 100 nm Glu-Plg with 0.25 nm tctPA without DNA (●) and with 1.0 (○) and 25 (□) μg/ml dsDNA in 0.05 m Hepes/NaOH buffer (pH 7.4) (with 20 mm NaCl and BSA 1 mg/ml). Inset, dependences of rates of fibrinolysis of FITC-fibrin film by 20 nm PL (Δ), mixtures of 100 nm Glu-Plg with 0.25 nm tctPA (●), or 0.25 nm tcuPA (○), 100 nm Plg/tctPA with 15 nm α2AP (■) and 100 nm Plg/tcuPA with 15 nm α2AP (□) on the concentration of dsDNA in the solution. The rates of fibrinolysis were calculated as slopes of a linear (r2 >0.9) increase in the fluorescein fluorescence emission with time (PL) or the square of time (tctPA/Plg and tcuPA/Plg) and expressed as the % of the control reaction (no DNA added). B, effects of dsDNA incorporated into a FITC-fibrin film on the rates of fibrinolysis by 20 nm PL (Δ) and a mixture of 100 nm Glu-Plg with 0.25 nm tctPA (●) or 0.25 nm tcuPA (○). The indicated amounts of dsDNA (μg/well) were added to 20 μg of FITC-Fbg before its polymerization. The rates of fibrinolysis were calculated as slopes of a linear (r2 >0.9) increase in the fluorescein fluorescence emission with time (PL) or the square of time (tctPA/Plg) and expressed as % of the control reaction (no DNA added). C, effects of oligo(dT)65 incorporated into FITC-fibrin film on the rates of fibrinolysis by 20 nm PL (Δ) and a mixture of 100 nm Glu-Plg with 0.25 nm tctPA (●) or 0.25 nm tcuPA (○). The indicated amounts of nanomoles of oligo(dT)65 per well were added to 20 μg of FITC-Fbg before its polymerization. Rates of fibrinolysis were calculated as slopes of a linear (r2 >0.9) increase in the fluorescein fluorescence emission with time (PL) or the square of time (tctPA/Plg) and expressed as % of the control reaction (no oligo(dT)65 added). D, effects of α2AP (15 nm) on the rates of fibrinolysis of FITC-fibrin films with incorporated dsDNA (circles) or oligo(dT)65 (squares) by 100 nm Plg/tctPA (filled symbols) and 100 nm Plg/tcuPA (open symbols). The indicated amounts of dsDNA (μg/well) or oligo(dT)65 (nanomoles/well) were added to 20 μg of FITC-Fbg before its polymerization. The rates of fibrinolysis were calculated as slopes of a linear (r2 >0.9) increase in the fluorescein fluorescence emission with the square of time and expressed as % of the control reaction (no dsDNA or oligo(dT)65 added).

Article Snippet: Custom oligonucleotides (oligo(dT) 20 ; oligo(dT) 65 , oligo(dAT) 10 (a 20-mer containing 10 AT repeats); oligo(dAT) 33 (a 66-mer containing 33 AT repeats); and TEX615-oligo(dAT) 33 , (an oligonucleotide labeled with red wavelength dye TEX615 at the 5′ end) were synthesized by Integrated DNA Technologies Inc. (Iowa City, IA).

Techniques: Fluorescence, Incubation, Concentration Assay